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AxyPrep Mag PCR Clean-up
The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental of the success or failure of down stream applications such as genotyping Sanger and next generation sequencing. The AxyPrep Mag PCR Clean-up kit utilizes a unique paramagnetic bead technology for quick high-throughput purification of PCR amplicons....
AxyPrep Mag DyeClean
Excess dye terminator clean-up post sequencing reaction clean-up is an essential step prior to sequencing. The carry over of excess dye into sequencing results in dye blobs which leads to overwhelming noise and failed calls of sequenced bases and potentially leading to the failure the sequencing run. AxyPrep Mag DyeClean...
AxyPrep Mag PCR Normalizer Kit
Core labs and sequencing service providers receives sequencing request for PCR products from various end users with variable concentration and conditions. What remains a processing challenge is to normalize these presumably clean PCR products. Although this process is essential for streamlining the sequencing workflow, it is time consuming. The AxyPrep...
AxyPrep Mag FragmentSelect Kits
Post DNA shearing, the library construction process for next generation sequencing mostly requires fragment selection regardless of the platform. Obtaining high recovery post fragment selection is becoming an important contributor for the reduction of sequencing bias. The AxyPrep FragmentSelect-I kit is optimized for Illumina next generation sequencing platforms and Life...
AxyPrep Mag Plasmid Kits
The AxyPrep Mag Plasmid purification kit utilizes a magnetic bead technology for high-throughput purification of plasmid DNA from E.coli cells. AxyPrep Mag Plasmid kit can also be used with fosmid and BAC vector-based constructs ranging from 5kb to 150kb. The system consists of steps of pelleting cells, resuspension, lysis, neutralization,...
AxyPrep Mag Blood Genomic DNA Kits
The AxyPrep Mag Blood gDNA for Blood and Serum DNA Isolation Kit utilizes paramagnetic bead technology to isolate genomic DNA from fresh or frozen whole blood containing various anticoagulants such as Citrate, EDTA, and Heparin. The protocol can be performed in both 96-well and single tube formats. The protocol consists...
AxyPrep Mag Tissue Genomic DNA Kits
The AxyPrep Mag Tissue gDNA Kit utilizes a magnetic bead-based technology to isolate genomic DNA from rodent tail tissues. This protocol provides instructions to extract DNA from fresh or frozen rodent tails. The protocol is performed in 96-well format and 1.5 mL. Purification begins by the addition of lysis buffer,...
AxyPrep Mag FFPE (DNA-RNA- miRNA) Kits
The AxyPrep Mag FFPE nucleic acid purification kit utilizes a magnetic beads-based technology to isolate nucleic acids (both DNA & RNA) from a maximum input of 10 mg of formalin-fixed, paraffin-embedded tissue. The protocol can be performed in both 96-well plates (manually and automated) and in 1.5 mL tubes. Nucleic...
AxyPrep™ Bacterial Genomic DNA Miniprep Kit
This kit is suitable for the rapid isolation of up to 20 mg of genomic DNA from 1.0×109 bacterial cells. Following lysis, separation of the genomic DNA from proteins, polysaccharides and lipids is achieved by a unique phase-partition step. The genomic DNA in the lower phase is selectively bound to...
AxyPrep™ Blood Genomic DNA Maxiprep Kit
The AxyPrep™ Blood Genomic DNA Maxiprep kits are designed to produce up to 100 mg and 500 mg of high molecular weight genomic DNA respectively from anticoagulated human or animal blood. Purification is based upon a unique phase-partitioning technology in combination with the selective binding of the genomic DNA to...
AxyPrep™ Blood Genomic DNA Midiprep Kit
The AxyPrep™ Blood Genomic DNA Midiprep kits are designed to produce up to 100 mg and 500 mg of high molecular weight genomic DNA respectively from anticoagulated human or animal blood. Purification is based upon a unique phase-partitioning technology in combination with the selective binding of the genomic DNA to...
AxyPrep™ Blood Genomic DNA Miniprep Kit
This kit is suitable for purifying up to 12 mg of genomic DNA from 250 ml of anti-coagulated whole blood. Blood genomic DNA is isolated directly from the white blood cell (WBC) component of whole blood, without the need to remove the red blood cells (RBCs) in advance. The isolation...
AxyPrep™ Body Fluid Viral DNA/RNA Miniprep Kit
This kit provides a rapid and highly reproducible way to purify viral nucleic acid from 250 ml of body fluid, including plasma, serum, ascites, cerebrospinal fluid, urine, cell culture supernatant, etc. During lysis, proteins and PCR inhibitors are removed by denaturation and precipitation. Viral nucleic acids remain soluble in the...
AxyPrep™ Multisource Genomic DNA Miniprep Kit
This kit is designed to purify genomic DNA from animal and plant tissues, cultured cells and yeast. Proteins, pigments, carbohydrates and lipids are efficiently segregated from the genomic DNA by a unique two-phase partition. The genomic DNA is bound to an AxyPrep™ Genomic DNA column, where residual impurities and salt...