Agarose LM (Low Melting)
- Vendor: Axygen
- Type: Molecular Biology Reagents
Agarose LM, low melting gels allows for the recovery of undamaged nucleic acids below their denaturation temperature. The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where In-Gel manipulations can be performed without prior extraction of the DNA from the gel slice. Low Melting (LM) Agarose is derivatized by organic synthesis which generates methoxylate groups from the basic agarose structure.
Applications
Agarose LM has the highest gelling/melting temperatures and gel strength.
- Electrophoresis of DNA fragments ≥ 1000 bp
- Tissue and cell culture
- Viral plaque assays
Agarose LM is also optimized for preparative electrophoresis and isolation of DNA fragments.
Specifications
| Clarity 1.5% (NTU) | ≤ 4 |
| ELECTROENDOSMOSIS (EEO) | 0.12 |
| SULFUR AS SO4 | 0.12% |
| GELLING TEMPERATURE (1.5 %) | 24-28° C |
| MELTING TEMPERATURE (1.5 %) | ≥65.5°C) |
| GEL STRENGTH (1.5 %) | ≥500 G/CM2 |
| DNASE | NONE DETECTED |
| RNASE | NONE DETECTED |
| DNA RESOLUTION ≥1000BP | FINELY RESOLVED |
Storage and Stability
Store in a cool dry place at 15‐25° Celcius.
Protocol: Electrophoresis of DNA and RNA
The most commonly used technique for DNA separation is electrophoresis in horizontal agarose gels
submerged in either Tris‐acetate or Tris‐borate buffer.
RNA molecules are separated in denaturing agarose gels containing formaldehyde.
RNA gels are submerged in MOPS buffer.
Step1: Add Agarose LE to a measured volume of buffer in a beaker or flask that is 3 times the
volume of solution prepared. The amount of Agarose LE added depends on the desired concentration.
Step2: Dissolve the Agarose by melting, simply by heating the slurry in a boiling water bath. If you
are using a microwave oven, heat on high power and mix gently.
Step3: Cool the solution to approx. 50°C before pouring.
Results
Agarose LM at different concentrations.
A-0.75%, B-1% and C-1.25%.
Marker: 1kb ladder, 0.5 μg/lane.
Running conditions: 1X TAE buffer, 4.5V/cm, 2 hours 30 min.
