Agarose MS (Molecular Screening)

 

Agarose MS, PCR screening, Molecular Biology Grade - 50g (AGR-MS-50)

$ 178.00 USD
 

Agarose MS, PCR screening, Molecular Biology Grade - 100g (AGR-MS-100)

$ 298.00 USD
 

Sample Agarose MS - 5gm/unit (AGR-SAM-MS)

$ 0.01 USD

Prices are in US Dollars and are valid for US Customers only.

Agarose MS is a molecular screening agarose for improved resolution of DNA fragments with 500 bp or less, especially primer–sized fragments.
At a 3 % concentration, Agarose MS gives a resolution of DNA fragments similar to gels made with polyacrylamide at concentrations of 8 %.

Features

  • Sharp finely resolved bands
  • High resolution of short PCR products and DNA fragments for improved clarity of the gel, enhancing visibility
  • Stronger gel structure and higher gel strength for better handling. The chances of gels breaking or cracking are greatly minimized, even at lower concentrations
  • High gel strength allows use in blotting
  • Low DNA binding for easy recovery

Applications

PCR screening

Specifications

Clarity 1.5% (NTU) ≤ 7
ELECTROENDOSMOSIS (EEO) 0.12
SULFUR AS SO4 0.11%
GELLING TEMPERATURE (1.5 %) ≥31° C
MELTING TEMPERATURE (1.5 %)   ≥76°C)
GEL STRENGTH (3 %)  ≥500 G/CM2
DNASE NONE DETECTED
RNASE NONE DETECTED

Ranges of Separation

3% 80 – 500 bp
4% 30 – 300 bp
5% 10 – 200 bp

These ranges are approximate and have been calculated in 1X TAE buffer.
To achieve the best resolution of Agarose MS gels, store at 4 ‐ 8°C for 20 hours before use.
Please note that while MS may be dissolved carefully by microwaving, gels are best prepared
by autoclaving.

Storage and Stability

Store in a cool dry place at 15-25° Celcius

Results

From the picture shown you can see the results given.

Agarose MS gel, 3% concentration in 1X TAE buffer.
Lane 1 – 250 bp ladder
Lane 2 – 100 bp ladder
Lane 3 – Molecular weight marker V (Roche)
Lane 4 – 10 bp ladder
Electrophoresis conditions: submarine gel, 2 hours, 4.5 V/cm in 1X TAE buffer.

Additional Material

Agarose MS