Agarose MS (Molecular Screening)
- Vendor: Axygen
- Type: Molecular Biology Reagents
Agarose MS is a molecular screening agarose for improved resolution of DNA fragments with 500 bp or less, especially primer–sized fragments.
At a 3 % concentration, Agarose MS gives a resolution of DNA fragments similar to gels made with polyacrylamide at concentrations of 8 %.
Features
- Sharp finely resolved bands
- High resolution of short PCR products and DNA fragments for improved clarity of the gel, enhancing visibility
- Stronger gel structure and higher gel strength for better handling. The chances of gels breaking or cracking are greatly minimized, even at lower concentrations
- High gel strength allows use in blotting
- Low DNA binding for easy recovery
Applications
PCR screening
Specifications
| Clarity 1.5% (NTU) | ≤ 7 |
| ELECTROENDOSMOSIS (EEO) | 0.12 |
| SULFUR AS SO4 | 0.11% |
| GELLING TEMPERATURE (1.5 %) | ≥31° C |
| MELTING TEMPERATURE (1.5 %) | ≥76°C) |
| GEL STRENGTH (3 %) | ≥500 G/CM2 |
| DNASE | NONE DETECTED |
| RNASE | NONE DETECTED |
Ranges of Separation
3% 80 – 500 bp
4% 30 – 300 bp
5% 10 – 200 bp
These ranges are approximate and have been calculated in 1X TAE buffer.
To achieve the best resolution of Agarose MS gels, store at 4 ‐ 8°C for 20 hours before use.
Please note that while MS may be dissolved carefully by microwaving, gels are best prepared
by autoclaving.
Storage and Stability
Store in a cool dry place at 15-25° Celcius
Results
From the picture shown you can see the results given.
Agarose MS gel, 3% concentration in 1X TAE buffer.
Lane 1 – 250 bp ladder
Lane 2 – 100 bp ladder
Lane 3 – Molecular weight marker V (Roche)
Lane 4 – 10 bp ladder
Electrophoresis conditions: submarine gel, 2 hours, 4.5 V/cm in 1X TAE buffer.
