<p>Agarose MS is a molecular screening agarose for improved resolution of <span class="caps">DNA</span> fragments with 500 bp or less, especially primer–sized fragments.<br />
At a 3 % concentration, Agarose MS gives a resolution of <span class="caps">DNA</span> fragments similar to gels made with polyacrylamide at concentrations of 8 %.</p>
<h2>Features</h2>
<ul>
<li>Sharp finely resolved bands</li>
<li>High resolution of short <span class="caps">PCR</span> products and <span class="caps">DNA</span> fragments for improved clarity of the gel, enhancing visibility</li>
<li>Stronger gel structure and higher gel strength for better handling. The chances of gels breaking or cracking are greatly minimized, even at lower concentrations</li>
<li>High gel strength allows use in blotting</li>
<li>Low DNA binding for easy recovery</li>
</ul>
<h2>Applications</h2>
<p><span class="caps">PCR</span> screening</p>
<h2>Specifications</h2>
<table>
<tr>
<td> Clarity 1.5% (<span class="caps">NTU</span>) </td>
<td> ≤ 7 </td>
</tr>
<tr>
<td> ELECTROENDOSMOSIS (<span class="caps">EEO</span>) </td>
<td> 0.12 </td>
</tr>
<tr>
<td> SULFUR AS SO4 </td>
<td> 0.11% </td>
</tr>
<tr>
<td> GELLING TEMPERATURE (1.5 %) </td>
<td> ≥31° C </td>
</tr>
<tr>
<td> MELTING TEMPERATURE (1.5 %) </td>
<td> ≥76°C) </td>
</tr>
<tr>
<td> GEL STRENGTH (3 %) </td>
<td> ≥500 G/CM2 </td>
</tr>
<tr>
<td> <span class="caps">DNASE</span> </td>
<td> NONE DETECTED </td>
</tr>
<tr>
<td> <span class="caps">RNASE</span> </td>
<td> NONE DETECTED </td>
</tr>
</table>
<h2>Ranges of Separation</h2>
<p>3% 80 – 500 bp<br />
4% 30 – 300 bp<br />
5% 10 – 200 bp</p>
<p>These ranges are approximate and have been calculated in 1X TAE buffer.<br />
To achieve the best resolution of Agarose MS gels, store at 4 ‐ 8°C for 20 hours before use.<br />
Please note that while MS may be dissolved carefully by microwaving, gels are best prepared <br />
by autoclaving.</p>
<h2>Storage and Stability</h2>
<p>Store in a cool dry place at 15-25° Celcius</p>
<h2>Results</h2>
<p>From the picture shown you can see the results given.</p>
<p>Agarose MS gel, 3% concentration in 1X <span class="caps">TAE</span> buffer.<br />
Lane 1 – 250 bp ladder<br />
Lane 2 – 100 bp ladder<br />
Lane 3 – Molecular weight marker V (Roche)<br />
Lane 4 – 10 bp ladder<br />
Electrophoresis conditions: submarine gel, 2 hours, 4.5 V/cm in 1X <span class="caps">TAE</span> buffer.</p>
<h2>Additional Material</h2>
<p><img src='/images/admin/icons/mimes/pdf.gif' alt='' /> <a href='http://static0.shopify.com/s/files/1/0001/7488/files/AgaroseMS_Insert_text.pdf'> Agarose MS </a></p>2008-12-15T17:56:33-08:00agarose-ms-molecular-screening4664752Molecular Biology Reagents2008-12-17T14:27:36-08:00Agarose MS (Molecular Screening)2009-09-10T10:41:50-07:00AxygenAgarose
Agarose MS is a molecular screening agarose for improved resolution of DNA fragments with 500 bp or less, especially primer–sized fragments.
At a 3 % concentration, Agarose MS gives a resolution of DNA fragments similar to gels made with polyacrylamide at concentrations of 8 %.
h2. Features
* Sharp finely resolved bands
* High resolution of short PCR products and DNA fragments for improved clarity of the gel, enhancing visibility
* Stronger gel structure and higher gel strength for better handling. The chances of gels breaking or cracking are greatly minimized, even at lower concentrations
* High gel strength allows use in blotting
* Low DNA binding for easy recovery
h2. Applications
PCR screening
h2. Specifications
| Clarity 1.5% (NTU) | ≤ 7 |
| ELECTROENDOSMOSIS (EEO) | 0.12 |
| SULFUR AS SO4 | 0.11% |
| GELLING TEMPERATURE (1.5 %) | ≥31° C |
| MELTING TEMPERATURE (1.5 %) | ≥76°C) |
| GEL STRENGTH (3 %) | ≥500 G/CM2 |
| DNASE | NONE DETECTED |
| RNASE | NONE DETECTED |
h2. Ranges of Separation
3% 80 - 500 bp
4% 30 - 300 bp
5% 10 - 200 bp
These ranges are approximate and have been calculated in 1X TAE buffer.
To achieve the best resolution of Agarose MS gels, store at 4 ‐ 8°C for 20 hours before use.
Please note that while MS may be dissolved carefully by microwaving, gels are best prepared
by autoclaving.
h2. Storage and Stability
Store in a cool dry place at 15-25° Celcius
h2. Results
From the picture shown you can see the results given.
Agarose MS gel, 3% concentration in 1X TAE buffer.
Lane 1 - 250 bp ladder
Lane 2 - 100 bp ladder
Lane 3 - Molecular weight marker V (Roche)
Lane 4 - 10 bp ladder
Electrophoresis conditions: submarine gel, 2 hours, 4.5 V/cm in 1X TAE buffer.
h2. Additional Material
<img src='/images/admin/icons/mimes/pdf.gif' alt='' /> <a href='http://static0.shopify.com/s/files/1/0001/7488/files/AgaroseMS_Insert_text.pdf'> Agarose MS </a>
2008-12-15T17:56:33-08:00manual45412079332deny1Agarose MS, PCR screening, Molecular Biology Grade - 50g1178.004664752trueAGR-MS-50trueAgarose MS, PCR screening, Molecular Biology Grade - 50g2008-12-17T17:45:03-08:002008-12-15T17:56:33-08:00manual45412317992deny1Agarose MS, PCR screening, Molecular Biology Grade - 100g2298.004664752trueAGR-MS-100trueAgarose MS, PCR screening, Molecular Biology Grade - 100g2008-12-17T17:45:03-08:002008-12-15T17:56:33-08:00manual45412318012deny1Sample Agarose MS - 5gm/unit30.014664752trueAGR-SAM-MStrueSample Agarose MS - 5gm/unit2008-12-17T17:45:03-08:002008-12-15T17:56:33-08:008659392146647522008-12-17T17:45:03-08:00http://cdn.shopify.com/s/files/1/0001/7488/products/AgaroseMS_Page_1_Image_0001.png?1268235731