This kit employs a special Binding Solution in combination with an AxyPrep spin column to achieve high selectivity and recovery of DNA fragments. This product is designed to purify DNA fragments longer than 75 bp from PCRs and other enzymatic reactions, with an expected recovery of 70-90%. Each column has a binding capacity of up to 8 µg. It is not necessary to remove mineral oil overlays from the PCRs before purification. This protocol will remove unincorporated primers (<50 nt), enzymes and unlabeled and fluorescent dye-labeled mononucleotides. The purified DNA fragments are suitable for a variety of applications, such as sequencing, ligation, restriction analysis, in vitro transcription, microinjection and microarrays.

Additional Materials:

• AxyPrep™ PCR Cleanup Kit brochure

• AxyPrep™ PCR Cleanup Kit protocol

MSDS

• AxyPrep™ PCR Cleanup Buffer PCR-A

• AxyPrep™ PCR Cleanup Buffer W2

• AxyPrep™ PCR Cleanup Eluent

Certificates of Analysis

• Click here to retrieve Certificates of Analysis

For volume discount pricing please contact Axygenbio at 888-5-AXYBIO or sales@axygenbio.com