<p>Recombinant Taq polymerase from Axygen Bioscience is a genetically modified for enhanced activity and stability at ambient temperature. The Taq is supplied in a kit complete with 10X Standard Taq Buffers with and without and magnesium chloride in separate vials.</p> <ul> <li>Highly pure Taq polymerase from a recombinant source.</li> <li>High activity for standard <span class="caps">PCR</span> applications; gives high yields.</li> <li>Stable at ambient temperature for a month.</li> <li>Supplied as a complete kit with 10X Reaction buffer with Mg+, 10X buffer Mg+ free buffer and MgCl2</li> <li>Exceptional value compared to high price brands.</li> </ul> <h2>Applications</h2> <ul> <li>Polymerase Chain Reaction (PCR)</li> <li>High-Throughput <span class="caps">PCR</span></li> <li>Primer Extension</li> <li><span class="caps">DHPLC</span></li> <li>Microarray Analysis</li> </ul> <h2>Reagents Supplied</h2> <ul> <li>Standard Taq Polymerase -500 units.</li> <li>Taq Reaction Buffer -1ml</li> <li>Taq Reaction Buffer-Mg2 Free -1ml</li> <li>Magnesium Chloride -1ml</li> </ul> <h2>Unit Definition</h2> <p>One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.</p> <p><strong>Concentration:</strong> 5 Units per μL.</p> <p><strong>Long Term Storage Temperature:</strong> -20°C<br />Stable for one month at ambient temperature without significant loss of activity.</p> <p><strong>Storage Buffer:</strong> 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.5% Tween 20, 0.1 mM <span class="caps">EDTA</span>, 1mM <span class="caps">DTT</span>, and 50% glycerol.</p> <p><strong>10X Taq Reaction Buffers:</strong> 500mM Tricine (pH 8.0), 120mM Potassium Acetate, 30mM Magnesium Sulphate, 0.1% Triton X-100; and 50μg/ml <span class="caps">BSA </span>(DNA and <span class="caps">RNA</span> free).</p> <p><strong>10X Taq Reaction Buffer Magnesium Free:</strong> Same as above but without Magnesium sulphate.</p> <p><strong>PCR Taq Master Mix:</strong> Premixed ready-to-use solution containing recombinant Taq with enhanced activity and temperature stability, dNTPs and buffers. Just add template primers and water and amplify.</p> <p><strong>Master Mix Composition:</strong> &#8211; Taq <span class="caps">DNA</span> polymerase &#8211; 5X Reaction Buffer containing 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20 &#8211; 7.5 mM MgCl2<br />- 1 mM dNTPs of each 200 μM dATP, 200 μM dCTP, 200 μM dGTP and 200 μM dTTP.</p> <p><strong>dNTP Mixture:</strong><br />This ready-to-use mix consists of dATP, dCTP, dGTP and dTTP (each at a final concentration of 20 mM) in TE buffer, within one vial. The total concentration is 80 mM.</p> <p><strong>dNTP Set:</strong> Contains of 100 mM solutions of dATP, dCTP, dGTP and dTTP each in separate vials in TE buffer.</p> <h2>Additional Material</h2> <p><img src='/images/admin/icons/mimes/pdf.gif' alt='' /> <a href='http://static1.shopify.com/s/files/1/0001/7488/files/Axygen_Taq_Data.pdf'>Taq Polymerase Information</a></p> 2008-12-22T14:11:32-08:00 taq-polymerase 4746762 Molecular Biology Reagents 2008-12-22T14:21:03-08:00 2008-12-22T14:26:22-08:00 Axygen PCR Reagents Recombinant Taq polymerase from Axygen Bioscience is a genetically modified for enhanced activity and stability at ambient temperature. The Taq is supplied in a kit complete with 10X Standard Taq Buffers with and without and magnesium chloride in separate vials. * Highly pure Taq polymerase from a recombinant source. * High activity for standard PCR applications; gives high yields. * Stable at ambient temperature for a month. * Supplied as a complete kit with 10X Reaction buffer with Mg+, 10X buffer Mg+ free buffer and MgCl2 * Exceptional value compared to high price brands. h2. Applications * Polymerase Chain Reaction (PCR) * High-Throughput PCR * Primer Extension * DHPLC * Microarray Analysis h2. Reagents Supplied * Standard Taq Polymerase -500 units. * Taq Reaction Buffer -1ml * Taq Reaction Buffer-Mg2 Free -1ml * Magnesium Chloride -1ml h2. Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. *Concentration:* 5 Units per μL. *Long Term Storage Temperature:* -20°C Stable for one month at ambient temperature without significant loss of activity. *Storage Buffer:* 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.5% Tween 20, 0.1 mM EDTA, 1mM DTT, and 50% glycerol. *10X Taq Reaction Buffers:* 500mM Tricine (pH 8.0), 120mM Potassium Acetate, 30mM Magnesium Sulphate, 0.1% Triton X-100; and 50μg/ml BSA (DNA and RNA free). *10X Taq Reaction Buffer Magnesium Free:* Same as above but without Magnesium sulphate. *PCR Taq Master Mix:* Premixed ready-to-use solution containing recombinant Taq with enhanced activity and temperature stability, dNTPs and buffers. Just add template primers and water and amplify. *Master Mix Composition:* - Taq DNA polymerase - 5X Reaction Buffer containing 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20 - 7.5 mM MgCl2 - 1 mM dNTPs of each 200 μM dATP, 200 μM dCTP, 200 μM dGTP and 200 μM dTTP. *dNTP Mixture:* This ready-to-use mix consists of dATP, dCTP, dGTP and dTTP (each at a final concentration of 20 mM) in TE buffer, within one vial. The total concentration is 80 mM. *dNTP Set:* Contains of 100 mM solutions of dATP, dCTP, dGTP and dTTP each in separate vials in TE buffer. h2. Additional Material <img src='/images/admin/icons/mimes/pdf.gif' alt='' /> <a href='http://static1.shopify.com/s/files/1/0001/7488/files/Axygen_Taq_Data.pdf'>Taq Polymerase Information</a> 2008-12-22T14:11:32-08:00 manual 454 12590922 deny 1 Taq Polymerase - 500 Units 1 45.00 4746762 true PCR-TAQ-R-5 true 2008-12-22T14:26:22-08:00 2008-12-22T14:11:32-08:00 manual 454 12595142 deny 1 Taq Polymerase - 1000 Units 2 90.00 4746762 true PCR-TAQ-R-10 true 2008-12-22T14:26:22-08:00 2008-12-22T14:11:32-08:00 manual 454 12595162 deny 1 Taq Master Mix -5X - 1ml 3 163.00 4746762 true PCR-TAQ-MX-1 true 2009-08-24T10:28:04-07:00 2008-12-22T14:11:32-08:00 manual 454 12595182 deny 1 dNTPset (25umol/dNTP, 100mM) - 25μmolX4 4 163.00 4746762 true PCR-DNTP-S-254 true 2008-12-22T14:26:22-08:00 2008-12-22T14:11:32-08:00 manual 454 12595202 deny 1 dNTP mixture (80mM total conc. 20mM each dNTP) - 100μl 5 163.00 4746762 true PCR-DNTP-M-100 true 2008-12-22T14:26:22-08:00 2008-12-22T14:11:32-08:00 manual 454 12595232 deny 1 dUTP (25umol, 100mM) - 25μmol 6 45.00 4746762 true PCR-DUTP-R-25 true 2008-12-22T14:26:22-08:00 2008-12-22T14:11:32-08:00 8741742 2 4746762 2008-12-22T14:26:22-08:00 http://cdn.shopify.com/s/files/1/0001/7488/products/Taq_Gel_1.png?1268276237 Title