Taq Polymerase
- Vendor: Axygen
- Type: Molecular Biology Reagents
Recombinant Taq polymerase from Axygen Bioscience is a genetically modified for enhanced activity and stability at ambient temperature. The Taq is supplied in a kit complete with 10X Standard Taq Buffers with and without and magnesium chloride in separate vials.
- Highly pure Taq polymerase from a recombinant source.
- High activity for standard PCR applications; gives high yields.
- Stable at ambient temperature for a month.
- Supplied as a complete kit with 10X Reaction buffer with Mg+, 10X buffer Mg+ free buffer and MgCl2
- Exceptional value compared to high price brands.
Applications
- Polymerase Chain Reaction (PCR)
- High-Throughput PCR
- Primer Extension
- DHPLC
- Microarray Analysis
Reagents Supplied
- Standard Taq Polymerase -500 units.
- Taq Reaction Buffer -1ml
- Taq Reaction Buffer-Mg2 Free -1ml
- Magnesium Chloride -1ml
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Concentration: 5 Units per μL.
Long Term Storage Temperature: -20°C
Stable for one month at ambient temperature without significant loss of activity.
Storage Buffer: 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.5% Tween 20, 0.1 mM EDTA, 1mM DTT, and 50% glycerol.
10X Taq Reaction Buffers: 500mM Tricine (pH 8.0), 120mM Potassium Acetate, 30mM Magnesium Sulphate, 0.1% Triton X-100; and 50μg/ml BSA (DNA and RNA free).
10X Taq Reaction Buffer Magnesium Free: Same as above but without Magnesium sulphate.
PCR Taq Master Mix: Premixed ready-to-use solution containing recombinant Taq with enhanced activity and temperature stability, dNTPs and buffers. Just add template primers and water and amplify.
Master Mix Composition: – Taq DNA polymerase – 5X Reaction Buffer containing 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20 – 7.5 mM MgCl2
- 1 mM dNTPs of each 200 μM dATP, 200 μM dCTP, 200 μM dGTP and 200 μM dTTP.
dNTP Mixture:
This ready-to-use mix consists of dATP, dCTP, dGTP and dTTP (each at a final concentration of 20 mM) in TE buffer, within one vial. The total concentration is 80 mM.
dNTP Set: Contains of 100 mM solutions of dATP, dCTP, dGTP and dTTP each in separate vials in TE buffer.
